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Image Search Results
Journal: mAbs
Article Title: Assessment of the role of afucosylated glycoforms on the in vitro antibody-dependent phagocytosis activity of an antibody to Aβ aggregates
doi: 10.1080/19420862.2020.1803645
Figure Lengend Snippet: Antibody-mediated Aβ uptake in human cell systems. (a-c) Mean number of Fc Receptors determined using the QuantiBRITE PE kit on two different human macrophage preparations derived from human peripheral CD14+ monocytes (A, n = 1–2) and on the human THP-1 cell line (B, n = 2). Mean ± S.E.M. are provided. (c) Kifunensine high mannose sample (HM-Afuc) has similar dose-dependent Aβ phagocytosis activity as the standard mediated by primary human macrophages (two-way ANOVA for standard vs sample, p >.05; n = 3). Results are presented as the relative ADCP fluorescence unit (RFU) as measured using the trypan blue exclusion method versus antibody concentration. Mean ± S.E.M. and non-linear regression curve fit analysis are shown. (d) HM-Afuc has similar dose-dependent Aβ phagocytosis activity as the standard mediated by the THP-1 cell line (two-way ANOVA for standard vs sample, p >.05; n = 4). Samples were run in duplicate and Aβ-GFP phagocytosis was quantitated by flow cytometry. The mean ± S.E.M. of the percent positive GFP cells of 1000 cells sampled per sample and non-linear regression curve-fit analysis are shown.
Article Snippet:
Techniques: Derivative Assay, Activity Assay, Fluorescence, Concentration Assay, Flow Cytometry